In this case study we'll take a closer look at how correcting for higher-order aberrations can dramatically improve the resolutions depending on the characteristics of your sample.
Title
Value
Title
Description
Leave empty
Directory
Create Workspace
Enable
Navigate to the project directory:
Make a new directory called
imports
and navigate into it:
Create a symbolic link from the source data into the
imports
directory:
Parameter
Value
Absolute path to .csg file
Parameter
Value
Volume data path
Inputs
Source
Particle stacks
Particles from the Import Result Group
Initial volume
Volume from Import 3D Volumes
Parameter
Value
Symmetry
Initial lowpass resolution (A)
Minimize over per-particle scale
Enabled
Launch this job in parallel with the previous Homogeneous Refinement.
